SPIDER SILK PROTEINS AND METHODS FOR PRODUCING SPIDER SILK PROTEINS

摘要

The invention provides an isolated major ampullatespidroin protein, which consists of from 150 to 420 amino acidresidues and is defined by the formula REP-CT. REP is arepetitive, N-terminally derived protein fragment having from80 to 300 amino acid residues. CT is a C-terminally derivedprotein fragment having from 70 to 120 amino acid residues.The invention further provides an isolated fusion proteinconsisting of a first protein fragment, which is a majorampullate spidroin protein, and a second protein fragmentcomprising a fusion partner and a cleavage agent recognitionsite. The first protein fragment is coupled via said cleavageagent recognition site to the fusion partner. The inventionalso provides a method of producing a major ampullate spidroinprotein and polymers thereof.