首页> 外国专利> CARRIER FOR DETECTING FOODBORNE-ILLNESS-CAUSING BACTERIA, KIT FOR DETECTING FOODBORNE-ILLNESS-CAUSING BACTERIA, METHOD FOR DETECTING FOODBORNE-ILLNESS-CAUSING BACTERIA, AND PCR REACTION SOLUTION FOR FOODBORNE-ILLNESS-CAUSING BACTERIA

CARRIER FOR DETECTING FOODBORNE-ILLNESS-CAUSING BACTERIA, KIT FOR DETECTING FOODBORNE-ILLNESS-CAUSING BACTERIA, METHOD FOR DETECTING FOODBORNE-ILLNESS-CAUSING BACTERIA, AND PCR REACTION SOLUTION FOR FOODBORNE-ILLNESS-CAUSING BACTERIA

机译:用于检测引起食物生菌的细菌的载体,用于检测引起食物生菌的细菌的试剂盒,用于检测引起食物生病的细菌的方法以及用于检测引起细菌生菌的细菌的PCR反应溶液

摘要

This invention can, with high specificity, detect the presence or absence of Escherichia coli and Vibrio parahaemolyticus regardless of pathogenicity while simultaneously detecting the presence or absence of pathogenic Escherichia coli and Vibrio parahaemolyticus. This invention also makes it possible to detect Escherichia coli, Salmonella sp., Staphylococcus aureus, and Vibrio parahaemolyticus in a sample simultaneously and with high specificity. Said bacteria are detected by the use of a carrier, for detecting foodborne-illness-causing bacteria, on which the following are immobilized: three or more probes selected from the pyrH, vtx1, and vtx2 genes of Escherichia coli; one or more probes selected from the invA gene of Salmonella sp.; one or more probes selected from the dnaJ gene of Staphylococcus aureus; and four or more probes selected from the toxR, tdh, trh1, and trh2 genes of Vibrio parahaemolyticus.
机译:本发明可以高特异性地检测大肠杆菌和副溶血性弧菌的存在与否,而与致病性无关,同时检测致病性大肠杆菌和副溶血性弧菌的存在与否。本发明还使得可以同时且高特异性地检测样品中的大肠杆菌,沙门氏菌,金黄色葡萄球菌和副溶血弧菌。所述细菌通过使用载体进行检测,所述载体用于检测引起食源性疾病的细菌,其上固定有以下物质:三种或多种选自大肠杆菌的pyrH,vtx1和vtx2基因的探针;从沙门氏菌属物种的invA基因中选择的一种或多种探针;一种或多种选自金黄色葡萄球菌的dnaJ基因的探针;以及四个或更多个选自副溶血性弧菌的toxR,tdh,trh1和trh2基因的探针。

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