Systems and methods for quantitative assessment of autism spectrum disorder based on detected responses of a subject to displayed animations are disclosed. In one example approach, a method for performing an autism assessment of a subject includes presenting as animation on a display, tracking the subject's eye gaze location on the display during specific scenes occurring within the animation where the specific scenes are associated with an autism construct, calculating an amount of time the subject's eye gaze tracks to predetermined regions on the display within the specific scenes to obtain a calculated eye gaze time associated with the specific scenes and the autism construct, and outputting an indication of the calculated eye gaze time. Animations may be selected to target a plurality of autism constructs and detected responses may be used to generate response mappings across the plurality of autism constructs.
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