APPLICATION OF PEPTIDYLARGININE DEIMINASE 1 IN PREPARATION OF REAGENT FOR CLINICAL DIAGNOSIS OF TUMORS

摘要

An application of peptidylarginine deiminase 1 in preparation of a reagent or detection object for clinical diagnosis of tumors, wherein the tumors comprise uterine cancers, liver cancers, gastric cancers, esophageal cancers, ovarian cancers and lung cancers. In the specific application, an antibody against peptidylarginine deiminase 1 is prepared by a conventional method, and a qualitative or quantitative method and a matching kit for detecting peptidylarginine deiminase 1 are established. A blood diagnosis reagent for diagnosing tumors comprises a peptidylarginine deiminase 1 antibody, an HRP-IgG antibody and conventional reagents in the blood diagnosis reagent, wherein the conventional reagents in the blood diagnosis reagent comprise a carbonate buffer solution, a PBST lotion, a blocking solution, a developing solution and a stop solution. Experiments prove that PAD1 can be applied as a tumor blood marker, and PAD1 has feasibility as the tumor marker.