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A PRIMER FOR PCR AMPLIFICATION LINKED WITH COMPLEMENTARY SEQUENCES OR COMPLEMENTARY SEQUENCES INCLUDING MIS-MATCHED NUCLEOTIDES AND NUCLEIC ACID AMPLIFICATION METHOD USING THE SAME
A PRIMER FOR PCR AMPLIFICATION LINKED WITH COMPLEMENTARY SEQUENCES OR COMPLEMENTARY SEQUENCES INCLUDING MIS-MATCHED NUCLEOTIDES AND NUCLEIC ACID AMPLIFICATION METHOD USING THE SAME
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机译:与扩增序列或包含错配核苷酸的互补序列或互补序列相关的互补序列的PCR扩增底漆
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摘要
The present invention relates to a primer for PCR obtained by, directly or by using inosine as a linker, linking a complementary nucleotide sequence or a complementary nucleotide sequence including a mismatched nucleotide sequence to the 5-terminus of a forward or reverse primer; and to a PCR method comprising a step of mixing a nucleic acid template in a PCR composition containing the primer and then performing PCR on the mixture. The primer for PCR of the present invention comprises a complementary nucleotide sequence or a mismatched nucleotide sequence in a complementary nucleotide sequence, which is linked to the 5-terminus thereof directly or via a linker, thereby lowering the sensitivity increase due to the increase in amplification products and reducing non-specifically occurring reactions in PCR.
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