DNA Methods for Detection Methylation on Promoter and Methods for Detection and Tracking Epigenetics Alterations by DNA methylation Using Nanoplasmonic Biosensor

摘要

The present invention relates to a method for detecting methylation on a promoter using a nanoplasmonic biosensor and a method for detecting and tracking the following genetic mutation by DNA methylation. More particularly, the present invention relates to a method for detecting methylation of a CpG region on a promoter using a nanoplasmonic biosensor As well as a method for evaluating the inhibition of transcription factor steric hindrance and epigenetic mediated transcription due to CpG site methylation, as well as methods for the detection and methylation of CpG. The methylation analysis method of the promoter using the nanoplasmonic biosensor of the present invention can measure the methylation status and the number of the CpG site immediately without pretreatment such as bisulfate treatment and simultaneously measure the methylation status of the p53 promoter and the overexpressed MBD2 And can be applied and applied to early cancer diagnosis. In addition, since the transcriptional activity change due to the methylation of the promoter can be measured, the transcriptional activity according to the epigenetic mediator can be tracked and evaluated.