- - ratiometric two-photon fluorescent probes for -galactosidase synthesis method of the same and quantitative imaging method of senescence-associated -galactosidase in vivo using the same

摘要

The present invention relates to a two-photon fluorescence probe, which is characterized by a two-photon fluorescence probe represented by any one of Chemical Formulas 1 to 4, wherein the two-photon fluorescence probe according to the present invention has high solubility in water, no toxicity, Therefore, the activity of beta-galactosidase can be effectively measured in vivo. In addition, it has remarkably superior specificity and luminescence intensity compared with the dyeing method of measuring the activity of beta-galactosidase in the prior art, and has excellent imaging ability in vivo, so that it can diagnose the aging step, For the diagnosis of diseases, diagnostic reagents and therapeutic agents.