The recombinant protein DMA-CBD, its production method, a recombinant plasmid pDMA-CBD and the strain E. coli M15 for its preparation, an injectable drug to increase muscle mass of farm animals, birds and animals canines, as well as the method of use of the drug

摘要

1. Recombinant protein DMA-CBD with a molecular weight (25,73 ± 1000) Yes pI 5,05, DMA protein comprising a fragment of sequence SEQ ID NO 1, Gly-Ser spacer to SEQ ID NO 2 sequence tsellyulozosvyazuyuschy domain endoglucanase gene CelD from A. thermophilum sequence with SEQ ID NO 4.2. The process for producing DMA-CBD on the cellulose recombinant protein comprising: growing cells of the strain E. coli M15 [pREP4, pDMA-CBD], DMA-CBD binding protein in cell extracts of E. coli M15 [pREP4, pDMA-CBD] Cellulosic sorbent due affinity interactions by incubation procedure, subsequent washing of unbound bacterial proteins ivydelenie produkta.3 target. The recombinant plasmid pDMA-CBD with the nucleotide sequence SEQ ID NO 4 provides DMA-CBD expression of a recombinant protein consisting of A determinants myostatin, Gly-Ser spacer domain endoglucanase gene tsellyulozosvyazuyuschego CelD from Anaerocellum thermophilum (A. thermophilum), size of 4091 n. n, consisting of the following structural elements: a. an artificial bacterial operon DMA-CBD recombinant protein comprising the promoter region of an early promoter of bacteriophage T5 7-87 bp DMA-CBD gene recombinant protein 115-813 bp nontranslated termination region trance RTOC 1693-1791 bp bacterial operon beta-lactamase, providing resistance to ampicillin 3886-3029 bp complementary strand, a bacterial origin of replication site type ColE1 2263-2273 bp provides plasmid replication in strains of E. coli.4. The strain of E. coli M15 [pREP4, pDMA-CBD], obtained by transformation with the plasmid of claim 3, deposited in the Russian Collection of Microorganisms under number VKM B-2880D.5. The injection for increasing muscle mass of farm animals, poultry and animals canine containing the recombinant protein DMA-CBD of claim 1, suspended in a medium of amorphous cellulose to 90% by weight, acceptably