RNA INTERFERENCE OF THE RADIATOR PHYA1 GENE INCREASES THE QUALITY OF FIBERS, ELIMINATION OF ROOT, FLOWERING, RIPPING, AND POTENTIAL OF YIELD IN THE BOWLER GOSSYPIUM (GOSSYPIUM HIRSUTUM L.)

摘要

1. An isolated or recombinant polynucleotide molecule comprising a DNA sequence encoding part of the hinge region of the PHYA1 polypeptide of a Gossypium hirsutum plant. 2. An isolated polynucleotide molecule according to claim 1, characterized in that said DNA sequence comprises a molecule of 213 consecutive nucleotide base pairs encoding a part of the hinge region of said PHYA1.3 polypeptide. An isolated polynucleotide molecule according to claim 1, characterized in that said DNA sequence is a sequence of SEQ ID NO: 1 or a sequence that is completely complementary to it. A hairpin nucleic acid construct encoding the gene sequence of the PHYA1 polynucleotide, comprising a portion of approximately 213 sequential sense nucleotide base pairs of the hinge region of the Gossypium PHYA1 gene and its antisense complement, so that the first and second polynucleotide sequences hybridize when they are transcribed into a double-nucleic acid ribonucleotide molecule. 5. The hairpin nucleic acid construct according to claim 4, characterized in that a part of the hinge region of 213 consecutive nucleotide base pairs is the sequence of SEQ ID NO: 1.6. A recombinant binary vector comprising the PHYA1 RNAi construct, characterized in that said construct comprises a nucleotide sequence of approximately 213 consecutive base pairs from the hinge region of the PHYA1 gene, with the 35S cauliflower mosaic virus (CaMV) promoter immediately before the PHYA1 hairpin , each construct is delivered by inoculation mediated by Agrobacterium, resulting in re