COMPOSITION OF AGRICULTURAL COATING FOR DETERMINING THE CONCENTRATION AND COMPOSITION OF THE POPULATION OF THE MACHUUPO VIRUS - AN EFFECT OF THE BOLIVIAN HEMORRHAGIC FEVER USING THE NEGATIVE COLONY METHOD

摘要

The composition of the agar coating for determining the concentration and composition of the Machupo virus, the causative agent of Bolivian hemorrhagic fever, using the method of negative colonies, which includes the following components in optimal concentrations: in the primary agar coating: amino acid vitamin complex (AVK), fetal calf serum, demineralized water , antibiotics, bactoagar "Difco"; in the secondary agar coating: Earl's solution, fetal calf serum, l-glutamine, sodium bicarbonate, demineralized water, neutral red, antibiotics, Difco bactoagar, characterized in that the claimed composition contains the following amounts of the presented components (by volume): primary agar coating amino acid vitamin complex (AVK) - 12.1%, fetal calf serum - 2.1%, demineralized water - 21.4% and antibiotics - 0.2%; Bacteriagar "Bacteriologi Cal" or "Bacto ™ Agar" - 50.0%; in the secondary agar coating: Earl's solution - 10.0%, fetal calf serum - 2.0%, sodium bicarbonate - 2.0%, demineralized water - 19.0%, neutral red - 5.0%, Bacteriologi Cal "Or" Bacto ™ Agar "- 25.0%.