DNA CODING FULL-SIZE HUMAN INTERLEUKIN-RECEPTOR ANTAGONIST 36, DNA CODING FULL-SIZE HUMAN INTERLEUKIN-RECEPTOR ANTAGONIST 36 WITH C-END POLIGISTIDINE TAG, PLASMID EXPRESSION VECTOR (VERSIONS), ESCHERICHIA COLI BL21 DE3 STAR (PET-IL36RAF),-PRODUCER OF FULL-SIZE RECEPTOR ANTAGONIST 36 AND STRAIN OF BACTERIA ESCHERICHIA COLI BL21 DE3 STAR (PET-IL36RAF-HIS),-PRODUCER OF FULL-SIZE RECEPTOR ANTAGONIST 36 WITH C-END POLIGISTIDINE TAG

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to biotechnology, specifically to recombinant production of proteins, and can be used for production of interleukin-36 receptor antagonist. Bacterial strain E.coli BL21Star [DE3] (pET-IL36Raf) - producer of full-size human interleukin-36 receptor antagonist is obtained by transformation of cells of E.coli parental BL21Star [DE3] with plasmid vector pET-IL36Raf. Bacterial strain E.coli BL21Star [DE3] (pET-IL36Raf-His) - producer of full-size human interleukin-36 receptor antagonist with C-end polyhistidine tag is obtained by transformation of cells of E.coli parental BL21Star [DE3] with plasmid vector pET-IL36Raf-His.;EFFECT: higher efficiency of recombinant production of IL-36Ra.;6 cl, 4 dwg, 1 tbl, 3 ex