NON-INVASIVE METHOD FOR SPECIFIC 3D DETECTION, VISUALIZATION AND / OR QUANTIFICATION OF A ENDOGENEOUS FLUOROPHORE SUCH AS MELANIN WITHIN A BIOLOGICAL TISSUE

摘要

Method for detection, quantification and/or visualization of an endogenous fluorophore, in a biological tissue, the method comprising the steps consisting in: a) acquiring, after multiphoton excitation, a set of successive three- dimensional or two-dimensional images providing information on the decrease over time in the fluorescence signals in the sample, b) determining by processing these images, by performing a linear regression of the logarithm of the fluorescence signals, the spatial distribution in the sample of the slopes of said linear regression, and c) generating information on the presence and/or the volume density or surface density of a fluorophore in the sample at least on the basis of this spatial distribution, the fluorophore being melanin.