LACTIC ACID BACTERIUM MUTATED INTO OBLIGATORY ANAEROBE, METHOD FOR CONSTRUCTING SAME, AND EXPRESSION VECTOR FUNCTIONING IN OBLIGATORY ANAEROBIC LACTIC ACID BACTERIUM

摘要

Provided are: an obligatory anaerobic lactic acid bacterium expected as being usable as a safe remedy for a disease under an anaerobic environment, such as solid tumor, and a gene transport support, which is no danger of exerting side effects in a normal tissue; a method for constructing the same; and an expression vector which is useful in the construction method. The aforesaid obligatory anaerobic lactic acid bacterium is characterized by having been artificially mutated from a facultative anaerobe into an obligatory anaerobe, and being further transformable by an expression vector carrying a gene, which expresses an active protein useful in treating a disease under an anaerobic environment, transferred thereinto. The aforesaid expression vector is characterized by being an expression vector capable of functioning in an obligatory anaerobic lactic acid bacterium, which contains a plasmid replication protein gene(Rep)originating in a lactobacillus, a secretion signal sequence (PslpA-SSartP) consisting of an s-layer gene promoter originating in a lactobacillus and a PrtP protein secretion signal originating in a lactobacillus, and one or more kinds selection marker genes. Thus, it is possible to provide a remedy for a disease under an anaerobic environment and a microbial gene transport support which are highly safe and excellent.