A biosensor comprises first and second molecular components and is capable of displaying non-protease enzyme activity in response to a binding event mediated by first and second binding partners of the biosensor. The first and second binding partners may bind each other directly or may both bind a target molecule. At least the first molecular component comprises an inhibited non-protease enzyme, whereby the binding event switches the enzyme from a catalytically inactive state to an active state. The second molecular component may comprise a protease that cleaves the first molecular component to release inhibition of the non-protease enzyme of first molecular component. Alternatively, the second molecular component may comprise a trap molecule that binds a bait molecule of the first molecular component to release inhibition of the non-protease enzyme of first molecular component.
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