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METHOD OF QUANTIFYING 8-HYDROXY-2'-DEOXYGUANOSINE
METHOD OF QUANTIFYING 8-HYDROXY-2'-DEOXYGUANOSINE
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机译:定量8-羟基-2'-脱氧鸟苷的方法
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摘要
PROBLEM TO BE SOLVED: To provide a method of quantifying 8-hydroxy-2'-deoxyguanosine, which enables high-sensitivity detection of low-concentration 8-hydroxy-2'-deoxyguanosine.;SOLUTION: A method comprises: feeding a solution containing 8-hydroxy-2'-deoxyguanosine into a first reverse-phase column 1; eluting 8-hydroxy-2'-deoxyguanosine from the first reverse-phase column using a water-based solvent; transferring only the eluted 8-hydroxy-2'-deoxyguanosine fraction to a second reverse-phase column 2; refining the 8-hydroxy-2'-deoxyguanosine in the second reverse-phase column; and measuring and quantifying the refined 8-hydroxy-2'-deoxyguanosine using an MS/MS/MS.;SELECTED DRAWING: Figure 1;COPYRIGHT: (C)2017,JPO&INPIT
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机译:解决的问题:提供一种定量8-羟基-2'-脱氧鸟苷的方法,该方法能够高灵敏度地检测低浓度的8-羟基-2'-脱氧鸟苷。 8-羟基-2'-脱氧鸟苷进入第一反相塔1;使用水性溶剂从第一反相柱上洗脱8-hydroxy-2'-deoxyguanosine;仅将洗脱的8-羟基-2'-脱氧鸟苷馏分转移至第二反相塔2;在第二个反相塔中精制8-羟基-2'-脱氧鸟苷;并使用MS / MS / MS测量和定量精制的8-羟基-2'-脱氧鸟苷;选定的图样:图1;版权:(C)2017,JPO&INPIT
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