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A BIOREACTOR MODULE, A BIOREACTOR SYSTEM AND METHODS FOR THICK TISSUE SEEDING AND CULTIVATION IN AN HIREARCHICAL ORGANIZATION AND PHYSIOLOGICAL MIMIKING CONDITIONS
A BIOREACTOR MODULE, A BIOREACTOR SYSTEM AND METHODS FOR THICK TISSUE SEEDING AND CULTIVATION IN AN HIREARCHICAL ORGANIZATION AND PHYSIOLOGICAL MIMIKING CONDITIONS
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机译:在分层组织和生理学模仿条件下进行厚组织播种和培养的生物反应器模块,生物反应器系统和方法
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摘要
The invention provides a bioreactor and methods for tissue cultivation. A bioreactor module comprises a container, a holder adapted to hold a scaffold containing an inherent vascular network, an inlet connectable to a vessel of the inherent vascular network, an inflatable device disposed within the container, and a pair of electrodes attached to opposing walls of the container, wherein the holder is removably receivable in the container and the inflatable device has a conduit extending through a wall of the container. An alternative embodiment provides an in-vitro method for tissue cultivation, comprising seeding an interior and an exterior of a vessel of an inherent vascular network of a scaffold with a first and a second cell type, respectively, and perfusing through the inherent vascular network with culture medium to facilitate compartmentalized co-cultivation of the first and the second cell type in different niches of the tissue. A further embodiment provides an in-vitro method for tissue cultivation, comprising seeding a surface of a scaffold with a predetermined cell type, and perfusing the scaffold with culture medium from an opposite surface of the scaffold through the scaffold and towards the seeded surface to create a nutrient/oxygen gradient and cause migratory diffusion induced penetration of cells towards the opposite surface.
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