COMPOSITIONS AND METHODS FOR GALLS FL AND GALLS CT MEDIATED TRANSFORMATION OF PLANTS

摘要

The present disclosure is directed to compositions and related methods that incorporate GALLS full-length (FL) or CT domain proteins to enhance efficiency of genetic manipulation of plants. In one aspect, the disclosure provides a modified Agrobacterium cell that comprises a first nucleic acid and a second nucleic acid that encodes a GALLS-FL protein. In another aspect, the disclosure provides a method of enhancing the single copy insertion of a first nucleic acid sequence into a plant cell genome. In another aspect, the disclosure provides a method of inducing plant susceptibility to Agrobacterium-mediated transformation, comprising providing GALLS-CT polypeptide in the cytosol of at least one cell of the plant. In another aspect, the disclosure provides a transgenic plant that comprises a heterologous nucleic acid sequence encoding GALLS-CT operably linked to a promoter sequence.