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Purification method for proteins, in particular antibodies, utilizing a wash solution comprising arginine at high pH for the affinity chromatography step
Purification method for proteins, in particular antibodies, utilizing a wash solution comprising arginine at high pH for the affinity chromatography step
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机译:蛋白质,特别是抗体的纯化方法,利用包含高pH值的精氨酸的洗涤液进行亲和层析步骤
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摘要
The invention provides a washing method for affinity chromatography in which a wash solution comprising arginine, or an arginine derivative, at pH greater than 8.0, is effective in removing impurities without the presence of a nonbuffering salt, while simultaneously increasing product concentration in the eluate and maintaining a high percent yield of recovered product.
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