Modified Yeast with lactate dehydrogenase encoding Gene of human origin with deletion of the gene encoding pyruvate decarboxylase

摘要

Yeast that can produce lactic acid with High Productivity without Neutralization with Alkali, characterized by a capability of lactic acid production and an excellent Growth capacity to produce, process and Method for production Go lactic acid in which it is used.Guest in the Yeast Schizosaccharomyces pombe have been introduced between 3 and 5 copies of a Gene that encodes a lactate dehydrogenase of human origin and has been deleted or Inactivated a Gene which is part of a group of genes encoding a pyruvate decarboxylase.Yeast which can be developed to reach a concentration of cells of at least 4.0 G (based on dry weight of cells / L) when it was cultivated in a Culture medium which was prepared by inoculating the cells at an initial concentration of 0,04 G (based on dry weight of cells / L) in a 500 ml Sakaguchi Flask containing 100 ml of Culture Broth Liquid comprising 1% Yeast Extract, 2% peptone and glucose 6% over a period of 20 hours at a temperature of 90 degrees, with agitation to go Speed of 110 rpm and at a depth of 7 cm.And can produce lactic acid in a concentration of at least 80 g / l when it is grown in a Culture medium which was prepared by inoculating the cells at an initial concentration of 0.04 G (based on dry weight of cells / L) in a 500 ml Flask containing Sakaguchi 100 ml of Culture Broth Liquid comprising 1% Yeast Extract,2% peptone and glucose 6% over a period of 20 hours at a temperature of 90 degrees, with stirring at a Speed of 110 rpm and at a depth of 7 cm, then inoculate the cells thus obtained at an initial concentration of 36 g (based on dry weight of algal cells. Cells / L) in a test tube with a diameter of 18 mm.And a length of 150 mm and contains 4.5 ml of an aqueous solution comprising 11.1% Glucose and carry out fermentation for a period of 3 hours at a temperature of 90 degrees, with an agitation with an angle of 43,5u00ba, at a Speed of 110 rpm and at a depth of 7 cm. Method for producing lactic acid which is used as a Yeast that has been described.