Methods for distinguishing, separating and classifying pollen grains containing one or more genetic elements of interest in one or more pollen grains, to determine the viability of one or more pollen grains, to identify plants with a genetic element of interest and for identific The air a genotype of a pollen grain

摘要

Methods for distinguishing, separating and classifying pollen grains containing one or more genetic elements of interest in one or more pollen grains.To determine the viability of one or more pollen grains to identify plants with a genetic element of interest and to identify a genotype of a pollen grain, the present invention relates to a method for classifying pollen containing genetic elements of interest that And can include associating a genetic marker with seeds and genetic elements of interest.To cultivate plants seeds, collect pollen, seeds, evaluate the pollen for the presence of genetic markers and classify the pollen based on the presence or absence of genetic markers. A method of determining the viability of the pollen may evaluate the optical density of the pollen grains and comparing the optical density with a limit of optical density.In addition, a mu00e9todio to identify plants with a genetic element of interest may include associating a genetic marker with the seed by defining the genetic element of interest, to cultivate plants seeds and to evaluate the plants for the presence of genetic marker.In addition, a method for identifying a genotype of pollen may include assessing the pollen by means of imaging spectral and / or hiperespectrais, and compare the pattern wavelength with standards of known wavelength.