Method for the construction of a recombinant fungal host cell, of polinucleotu00eddeo construct, recombinant fungal host cell, and method of production of a polypeptide of interest.

摘要

1 / 1 for a summary u00e2u20acu0153mu00e3u2030todo construu00e3u2021u00e3u0192o cu00e3u2030lula stewardess fu00e3u0161ngica recombinant construct of polinucleotu00e3ufffddeo, cu00e3u2030lula stewardess fu00e3u0161ngica recombinant, and.Mu00e3u2030todo of produu00e3u2021u00e3u0192o a polipeptu00e3ufffddeo of interesseu00e2u20acufffd this invenu00e3u00a7u00e3u00a3o refers to mu00e3todos for construu00e3u00a7u00e3u00a3o a cu00e3lula fu00e3u00bangica recombinant host comprising one or more cu00e3u00b3pias a construct of polinucleotu00e3u00addeo integrated in its genome.The mu00e3todo comprising the transformau00e3u00a7u00e3u00a3o a cu00e3lula fu00e3u00bangica with a host of polinucleotu00e3u00addeo integrative construct comprising a first polinucleotu00e3u00addeo encoding a marker selecionu00e3vel, characterized the first polinucleotu00e3u00addeo,A regiu00e3u00a3o 5u00e2u20acu2122 nu00e3u00a3o translated the same and / or a riboswitch operatively connected u00e3u00a0 same comprises a u00e3u00adntron spliceosomal has 5 nucleotu00e3u00addeos or less between the su00e3u00adtio of ramificau00e3u00a7u00e3u00a3o and its su00e3u00adtio receiver; and a second polinucleotu00e3u00addeo encoding a polipep Tu00e3u00addeo of interest; as well as polinucleotu00e3u00addicos constructs from adequate,Cu00e3lulas host fungi and mu00e3todos of fabricau00e3u00a7u00e3u00a3o.