isolated antigen binding polypeptide construct, isolated polynucleotide or set of isolated polynucleotides, vector or set of vectors, isolated cell, pharmaceutical composition, use of the construct, method of treating a subject with a disease or disorder, method of obtaining a construct , method for preparing a construct, computer readable storage medium, method for producing a bispecific antigen binding polypeptide construct, and method for preparing an isolated antigen binding polypeptide construct

摘要

The present invention provides heterodimer pairs which may comprise a first heterodimer and a second heterodimer wherein each heterodimer comprises an immunoglobulin heavy chain or fragment thereof and an immunoglobulin light chain or fragment thereof. at least one of the heterodimers may comprise one or more amino acid modifications in the ch1 and / or cl domains, one or more amino acid modifications in the vh and / or v1 domains, or a combination thereof. The modified amino acids can be part of the interface between the light chain and the heavy chain and are usually modified to create the preferential pairing between each heavy chain and a desired light chain, so that when the two heavy chains and two light chains of the heterodimer pair are coexpressed in the cell, the heavy chain of the first heterodimer is preferably paired with one of the light chains instead of the other. Similarly, the heavy chain of the second heterodimer typically is preferably paired with the second light chain rather than the first.