A method of increasing the signal of antibodies recognizing 5-bromo-2'-deoxyuridine and/or 5-chloro-2'-deoxyuridine and/or 5-iodo-2'-deoxyuridine at sites of incorporation of these nucleosides into DNA for DNase I activity.

摘要

ufeffzpu016fsob to effectively increase the signal antibody rozeznu00e1vaju00edcu00edch 5 - bromo - 2u00b4 - deoxyuridin and / or 5 -] - 2u00b4 - deoxyuridin and / or 5 - jodo - 2u00b4 - deoxyuridin dna incorporation of nucleosides into dna sites for interaction dnu00e1zou 1 or dnu00e1zou 1 and exonukleu00e1zou iii.the way lies in the incubation samples after exposure to dnu00e1zy 1 or dnu00e1zy i and exonukleu00e1zy iii and antibodies in solution of formaldehyde and is usable both in the case that they are used systems based on two antibodies, systems based on antibody conjugated with various lu00e1t kami, for example. fluorochromy.