method of obtaining u0431u0438u043eu0434u0435u0433u0440u0430u0434u0438u0440u0443u0435u043cu044bu0445 biocompatible u0442u043au0430u043du0435u0438u043du0436u0435u043du0435u0440u043du044bu0445 sheets using fibroblast cultures

摘要

the invention can be used independently or in an integrated surgical treatment of patients with deep u043eu0436u043eu0433u043eu0432u044bu043cu0438 wounds, extensive u043fu043eu0441u0442u0442u0440u0430u0432u043cu0430u0442u0438u0447 u0435u0441u043au0438u043cu0438 u0440u0430u043du0435u0432u044bu043cu0438 defects of skin and soft tissue cover, with all of u043eu0441u0442u0435u043eu043cu0438u0435u043bu0438u0442u043eu043c with defect of soft tissue, as well as the long-term u043du0435u0437u0430u0436u0438 the trophic sores.the technical objective of the invention is to develop a method for obtaining u0442u043au0430u043du0435u0438u043du0436u0435u043du0435u0440u043du044bu0445 sheets constituting u0434u0432u0443u0445u0441u043bu043eu0439u043du0443u044e u0431u0438u043eu0438u043du0436u0435u043du0435u0440u043du0443u044e design, cat u043eu0440u0430u044f contains a substrate of u0431u0438u043eu0434u0435u0433u0440u0430u0434u0438u0440u0443u0435u043cu043eu0433u043e u043cu0430u0442u0440u0438u043au0441u0430 consisting of natural polysaccharides, u0445u0438u0442u043eu0437u0430u043du0430 and u043fu0435u043au0442u0438u043du0430 appendix u0430u043du0442u0438u043eu043au0441u0438u0434u0430u043du0442u0430 taxifolin oh, and performing mechanical and supportive function.the second layer made from skin cells, fibroblast, is an artificial analogue u0434u0435u0440u043cu0430u043bu044cu043du043eu0433u043e skin layer and reduces the risk of excessive scar a swatch and.the cellular material, which is a slurry of fibroblast cultures of 50u00d710u043au043bu0435u0442u043eu043a 1 cmb nutritional environment without adding u043au0441u0435u043du043eu0433u0435 u043du043du043eu0439 serum, is applied to the surface of the substrate in the form of suspension and u0430u0434u0433u0435u0437u0438u0440u0443u0435u0442u0441u044f surface u043cu0430u0442u0440u0438u043au0441u0430 u0446u0435u043du0442u0440u0438u0444u0443u0433u0438u0440u043eu0432u0430u043du0438u0435u043c in 200G (1500 rpm) for 5 to 8 minutes, etc. e. cells not under.as a u0430u043bu043bu043eu0433u0435u043du043du043eu0433u043e material recommended the use of human diploid fibroblast strain u0444u044du0447 2 / 09 (innovative patent no. 25091 from 15.12.2011) or else oh u0430u0440u0430u043au0442u0435u0440u0438u0437u043eu0432u0430u043du043du043eu0439 culture of skin cells or autologous cells derived from the patient.the design characteristics of the graft to provide the most favorable fit to the defect and exclude