Method for increasing catalytic activity of 1,3-propanediol oxidoreductase enzyme, microorganism able to produce mutated oxidoreductase, method for producing the enzyme and application

摘要

in the manner described structure change to native enzyme oksydoreduktazy 1,3 - propanodiolowej structure mutated isoenzyme, creation of genetically zrekombinowanych micro-organisms containing mutant gene for modified isoenzyme and the use of those rekombinantu00f3w for the production of the enzyme preparation of oksydoreduktazy 13 - propanodiolowej and microbiological synthesis of 1,3-propanediol. the modification oksydoreduktazy 1,3 - propanodiolowej is to change the chemical structure of enzyme by substitution of alanine at position 278 by walinu0119. alanine substitution for walinu0119 increases the catalytic activity of the enzyme.the mutation enzyme method error - prone pcr, and mutant enzyme (enzyme) increased activity reduction was determined by skriningu. the mutated gene coding for a modified dhat, oksydoreduktazu0119 1,3 - propanodiolowu0105, was introduced to the vector ekspresyjnego and wklonowany together with him to bacteria.in this way, the recombinant microorganisms containing vector sequence was mutated dhat isoenzyme. the recombinant microorganisms capable of producing modified structurally isoenzyme a278v, were used to express the mutated gene and synthesis of.the isolated and purified enzyme was the basis for the production of enzyme preparation, modified forms of oksydoreduktazy 1,3-propanediol. the recombinant microorganisms containing the mutated gene dhat, were used for the microbiological synthesis of 1,3-propanediol by fermentation of glycerol.in relation to gene recombinant bacteria containing non achieved increase production 1,3-propanediol by 5 - 50%. the object of the application is also a way of the mutated oksyreduktazy 1,3 - propanodiolowej and its application.