Method for neural stem cells and neural progenitor cells from embryonic no ovarian cortical tissue

摘要

The present invention relates to a method for obtaining neural stem cells and neural progenitor cells (NSC/NPC) from ovarian cortical tissue of pre-pubescent female mammals, without needing to add factors for inducing and/or expanding the neural line to the culture medium. The method of the invention can be used to obtain NSC/NPC after 8–21 days of incubation, improving the results by adding follicle stimulating hormone (FSH) to the culture medium. The NSC/NPC obtained using this method can be used in experimental models of in-vitro neurogenesis, in directed NSC/NPC differentiation to generate different types of nerve cells of interest in medicine for regenerating the nervous system, with use in autologous transplantation in cell therapy of neurodegenerative diseases, and to evaluate the neurotoxicity of different drugs, contaminants and chemical compounds.