GENE TRANSFER INTO AIRWAY EPITHELIAL STEM CELL BY USING LENTIVIRAL VECTOR PSEUDOTYPED WITH RNA VIRUS OR DNA VIRUS SPIKE PROTEIN

摘要

A gene is successfully transferred into airway epithelial stem cells by usinga monkey immunodeficiency virus vector having been pseudotyped with Sendaivirus envelope glycoproteins F and HN. This gene transfer into airwayepithelial stem cells with the use of the vector as described above is usefulin gene therapy for a hereditary respiratory disease such as cystic fibrosis.Moreover, it becomes possible to select a respiratory organ such as the lungas a tissue that is capable of producing and supplying a protein deficient ina hereditary disease.