METHOD FOR INVESTIGATING BLOOD-BRAIN BARRIER PROPERTIES AND BRAIN METABOLISM USING INSECT BRAINS

摘要

The present disclosure relates to a method for investigating blood-brain barrier properties and brain metabolism using an insect brain as a model system. The method comprises dissecting out an insect brain comprising the compound eyes, and exposing the brain and compound eyes to a medium comprising a chemical compound. The method allows for the investigation of various properties, including blood-brain barrier permeability of compounds, the effect agents have on the blood-brian barrier, and various aspects of brain chemistry including distribution and metabolism of agents within the brain. The method finds various uses, such as an in vitro screening model as part of drug development, or as a methodology to investigate the impact of various agents on brain chemistry and the integrity of the blood-brain barrier, both in vitro and in vivo.