METHOD FOR THE DIFFERENTIATION OF ESCHERICHIA COLI AND SHIGELLA BACTERIA BY MEANS OF NMR SPECTROMETRY

摘要

The invention relates to a method for the proton NMR spectrometry-based differentiation of Escherichia colibacteria and Shigella bacteria, said method comprising the steps of: obtaining at least one supernatant of at least one suspension of bacteria that could be Escherichiacoli or Shigella; preparing at least one sample suitable for proton NMR spectrometry analysis, by mixing at least one fraction of at least one supernatant with a deuterated buffer; obtaining at least one NMR spectrum of the at least one sample; analysing the peaks of the at least one spectrum corresponding to the following metabolites: acetate, alanine, aspartate, ethanol, lactose, lysine, Na-acetyllysine, propionate, serine, succinate, threonine and valine; determining if it is Escherichiacoli or Shigella bacteria.