METHODS FOR SEPARATING AND CULTURING HUMAN TESTICULAR MESENCHYMAL STEM CELLS, AND USE OF HUMAN TESTICULAR MESENCHYMAL STEM CELLS

摘要

Provided is a method for separating human testicular mesenchymal stem cells. The method comprises: digesting a testis sample, separated out by means of biopsy, of an obstructive azoospermia patient with type IV collagenase, and filtering to remove tissue lumps; and after human testicular cells are obtained, using a CD51 antibody to label the testicular cells, then sorting by using a flow cytometry, separating and collecting the human testicular mesenchymal stem cells which are positive in CD51. Also provided are a method for culturing human testicular mesenchymal stem cells, and use of human testicular mesenchymal stem cells in medicines for treating related diseases caused by low testosterone level.