E E C- C- A expression vector for expressing heavy chain C-terminal region protein comprisinga codon-optimized botulinumtoxin E type polynucleotide fragmentation a transgenic cell transformed with the vector and a preparation method of heavy chain C-terminal region protein using the same

摘要

The present invention provides a vector for expressing a heavy chain C-terminal region protein of Bolulenum toxin type E, which comprises the SV40 enhancer, the CMV promoter and the polynucleotide fragment of codon-optimized bovulin enterotoxin E, which are sequentially connected, Cell region and a method for producing the heavy chain C-terminal region protein of Botulinum Toxin Type E using the same. More particularly, the present invention relates to a method for producing a heavy chain C-terminal region protein having an increased amount of an antigen and a bacterial- .