METHOD FOR QUALITATIVE AND QUANTITATIVE DETECTION OF COMMON WHEAT

摘要

The present invention provides a method for specifically, highly sensitive, qualitatively and / or quantitatively detecting a bismuth wheat among wheat contained in various tested samples such as food raw materials and processed foods. The present invention provides a method for qualitatively and / or quantitatively determining and detecting a wheat, for example, a durum wheat, other than the beef staple wheat and the beef staple wheat contained in a food raw material or a processed food. A primer set, a nucleic acid probe, and a detection kit which can be used for the detection method using these PCR methods are provided. As a method for detecting the presence of a bismuth wheat in a test sample, a method of detecting the presence of a bismuth wheat in a test sample is described, wherein a nucleic acid extracted from a test sample is used as a template and a primer having a nucleotide sequence shown in SEQ ID NO: 5 and a nucleotide sequence having a nucleotide sequence shown in SEQ ID NO: Performing PCR using a primer and detecting the presence of a PCR amplification product; As a method for detecting the presence of a bismuth wheat in a test sample, there is provided a method for detecting the presence of a bismuth wheat in a test sample, which comprises using as a template a nucleic acid extracted from a test sample, a primer having a nucleotide sequence shown in SEQ ID NO: 5, a primer having a nucleotide sequence shown in SEQ ID NO: A nucleic acid probe having the nucleotide sequence shown in SEQ ID NO: 11 is used to carry out quantitative PCR, and the presence of a bovine mast is detected qualitatively and / or quantitatively.