2 Polyvalent primers that detect two or more virus selected from spherical virus group consisting the genera Bromovirus and genera Cucumovirus

摘要

The present invention relates to virus-bromo (Bromovirus) in viruses and Cuckoo parent virus (Cucumovirus) virus in the group spherical virus diagnostic method using a diagnostic primer in the same time a selected combination of two or more viruses, and this in made. More specifically, the simultaneous diagnostic primer set of the present invention comprises a PCR primer pair shown in SEQ ID NO: 1 and 2, a reverse primer shown in SEQ ID NO: 3, and an adapter primer shown in SEQ ID NO: 4 and 5. The simultaneous diagnostic primer according to the present invention exhibits an excellent effect for detecting specifically two or more viruses belonging to the genus Bromovirus and the genus Cucumovirus with a specific high sensitivity.