Bcr-Abl METHOD FOR DETECTING SPLICE VARIANTS OF BCR-ABL FUSION GENE AND PCR COMPOSITION FOR DETECTING THEREOF

摘要

The present invention relates to a method for detecting a target conjugated mutant gene of a Bcr-Abl fusion gene and a polymerase chain reaction composition for detection, and a method for detecting a target conjugate gene of a Bcr-Abl fusion gene provided by the present invention is characterized by gel electrophoresis Accurate quantification can be used to quantify the level of mRNA expression of difficult mutant fusion genes, and using this method, it is possible to detect a target conjugated variant gene in a technically simple and cost-effective manner. Thus, the method of detecting a target junction variant gene of the present invention can be applied to early diagnosis and treatment plan of chronic leukemia by graphene-based fluorescence detection assay.