首页> 外国专利> Genetic Marker for Dectecting Spring Viraemia of Carp Virus and Method for Detecting Spring Viraemia of Carp Virus Using the Same

Genetic Marker for Dectecting Spring Viraemia of Carp Virus and Method for Detecting Spring Viraemia of Carp Virus Using the Same

机译:鲤病毒春季病毒血症的遗传标记检测方法及使用该方法的鲤病毒春季病毒血症的检测方法

摘要

The present invention relates to a genetic marker for detecting spring viraemia of carp virus (SVCV) and a method for detecting SVCV using the same and, more specifically, to a method for amplifying a specific nucleic acid of the SVCV, hybridizing a peptide nucleic acid (PNA) which specifically recognizes the amplified product, obtaining a temperature-dependent melting curve by controlling the temperature of the hybridized product, and detecting the SVCV or confirming whether a carp is infected with the virus from the melding temperature through the analysis of the obtained melting curve. According to the present invention, as the SVCV is detected simply, quickly and accurately, the infection of the virus recognized as the biggest threat to the carp culture and the spread of the spring viraemia of carp caused by the virus can be prevented early.
机译:本发明涉及一种用于检测鲤鱼病毒春季病毒血症的遗传标记物以及使用该基因标记物检测SVCV的方法,更具体地,涉及一种通过使肽核酸杂交来扩增SVCV的特定核酸的方法。 (PNA)可以特异性识别扩增产物,通过控制杂交产物的温度,获得SVCV,或者通过分析所得的融合温度,从融合温度中确定鲤鱼是否感染了病毒,从而获得温度相关的解链曲线熔解曲线。根据本发明,由于可以简单,快速,准确地检测出SVCV,因此可以及早预防被认为是对鲤鱼养殖的最大威胁的病毒感染和鲤鱼春季病毒血症的传播。

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