Cell for analyzing mitochondria in cell produced by cell nuclear transfer and method for providing information about mitochondria in cell produced by cell nuclear transfer

摘要

The present invention relates to a cell for analyzing a nuclear-substituted intracellular mitochondria, a method for producing the cell, and a method for providing information on a nuclear-substituted intracellular mitochondria. More particularly, the present invention relates to a method for providing information on a mitochondrial targeting sequence, The present invention relates to a cell for analyzing a nuclear-substituted intracellular mitochondria into which a protein-coding sequence-containing polynucleotide has been introduced, a method for producing the cell, and a method for providing information on nuclear-substituted intracellular mitochondria. In the nuclear-substituted cells prepared by transforming cells transfected with mitochondria-specific fluorescent proteins and tagged proteins according to the present invention, fluorescence and tagged proteins are continuously expressed specifically in mitochondria. Using the nucleus-substituted cells, information on the distribution, location, and reprogramming of oocytes and donor cells of the cytoplasm containing mitochondria during embryonic development can be obtained.;