HYALURONANLIASIS ENZYME, METHOD FOR ITS PREPARATION, ITS APPLICATION AND METHOD FOR PRODUCING LOW-MOLECULAR HYALURONANES

摘要

1. A method of producing hyaluronan lyase, characterized in that it is obtained by deep cultivation of a fungus belonging to the genus Fistulina, at a temperature of from 20 to 30 ° C for 5 to 11 days. The method according to claim 1, characterized in that the fungus belonging to the genus Fistulina is the species Fistulina hepatica. 3. A method according to claim 1 or 2, characterized in that the culture medium contains a carbon source, a nitrogen source and inorganic salts. The method according to claim 3, characterized in that the carbon source is sucrose, the nitrogen source is the yeast autolysate, and the inorganic salts are NaHPO · 12 HO and MgSO · 7HO.5. The method according to any one of paragraphs. 1, 2 or 4, characterized in that the enzyme is isolated from the culture medium after removal of the mycelium and / or by extraction from the mycelium after destruction of the mycelium. 6. A method according to claim 5, characterized in that the enzyme is washed at least once with a buffer with a pH of 7.0.7. A method according to claim 6, characterized in that the enzyme is further purified by chromatographic separation. Hyaluronan lyase obtained by the method according to claim 1 by deep cultivation in a culture medium of a fungus belonging to the genus Fistulina. 9. Hyaluronan lyase according to claim 8, characterized in that the fungus of the genus Fistulina is Fistulina hepatica. 10. Hyaluronan lyase according to claim 8 or 9, characterized in that it has optimal activity at pH 4.0 and at a temperature of 20 ° C. 11. The use of the hyaluronan lyase enzyme obtained by the method according to claim 1 by cultivating a fungus belonging to the genus Fistulina for the decomposition of hyaluronan or its derivative. The use of the hyaluronan lyase enzyme obtained by the method according to claim 1 by cultivating a fungus belonging to the genus Fistulina to obtain pharmaceutical or cosmetic