A cassette genetic construct expressing two biologically active siRNAs that effectively attack targets in HIV-1 subtype A reverse transcriptase mRNA in patients in Russia, and one siRNA directed to CCR5 gene mRNA

摘要

FIELD: medicine.SUBSTANCE: invention relates to medical and molecular genetics, and concerns cassette genetic construct. Presented cassette construct anti-HIV-1R-anti-HIV-2R-anti-CCR5-8 is obtained on basis of vector GeneClip-U1-neo and contains insert, expressing three hairpin-RNAs coding siRNA inhibitors of human immunodeficiency virus type 1 subtype A and human CCR5 gene expression and two fragments of genome of HIV-1 subtype A, found in Russian patients, and corresponding to two conservative areas of reverse transcriptase domain (are given in first and second lines respectively), as well as 19-bp area corresponding to CCR5 gene (is brought into third line):where lower case shows area of loops between parts of palindromes that can form double-stranded duplexes.EFFECT: invention can be used to suppress expression of versions of virus in Russian patients.1 cl, 3 dwg, 3 tbl, 2 ex