DIAGNOSTIC METHOD FOR SMC2 POLYMORPHISM ASSOCIATED WITH HH3 FERTILITY OF CATTLE FERTILITY

摘要

FIELD: biotechnology.SUBSTANCE: method of diagnostics of SMC2 polymorphism, associated with HH3 fertility haplotype of cattle stock is described. The method includes single tube allele specific amplification (STAS PCR) of SMC2 gene fragments, and differs in that the amplification products of allele T and C at position 95410507 (UMD 3.1 genome assembly) in exon of 24 gene of structural maintenance of SMC2 chromosomes vary in length. The identification of genotypes of animals is carried out according to the results of electrophoresis STAS PCR products in agarose gel.EFFECT: invention expands the arsenal of tools that allow for diagnostics of SMC2 polymorphism.2 dwg, 1 ex