Genetic (recombinant) DNA construct encoding the codon-optimized gene of rabies virus glycoprotein (protein G) with a consensus amino acid sequence, which is composed taking into account the amino acid sequences of protein G isolated from rabies virus strains circulating in the Russian Federation

摘要

FIELD: biotechnology.SUBSTANCE: genetic DNA construct represented by the nucleotide sequence SEQ ID NO 2 containing a codon-optimized gene encoding the glycoprotein (G protein) of rabies virus with the consensus amino acid sequence SEQ ID NO 1, taking into account the variety of the rabies virus strains circulating in the territory of the Russian Federation is proposed. The proposed DNA construct in the pVAX1 vector in the transfected human cell culture, provides an increase in protein G gene expression as compared to the expression of the gene of the Vnukovo-32 strain viral protein G in a similar vector.EFFECT: proposed DNA construct can be used to create an anti-rabies DNA vaccine that provides efficient glycoprotein synthesis in mammalian cells.2 dwg