METHOD OF DETERMINING CONTENT OF FREE GIBBERELLIC ACID IN VEGETATIVE PLANT ORGANS BY METHOD OF CAPILLARY ELECTROPHORESIS

摘要

FIELD: chemistry.;SUBSTANCE: invention can be used to determine the content of free gibberellic acid (GA₃) in vegetative plant organs of an apple, grapes, winter wheat. For this, samples of biological material are extracted with ethyl acetate, and then the extract is filtered and evaporated at 40°C. The dry residue is then dissolved in 60% aqueous acetone. Quantitative determination of free gibberellic acid content is carried out by capillary electrophoresis in a quartz capillary with effective length of 0.5 m and an internal diameter of 75 mcm with positive voltage polarity. A solution containing 0.33 wt % of boric acid, 0.05 wt % of sodium tetraborate and 0.5 vol. % of isopropanol, pH = 9.3 is used as an electrolyte. Detection is carried out at a wavelength of 254 nm.;EFFECT: quantitative determination of gibberellic acid in plant raw materials.;1 tbl, 3 dwg, 3 ex