METHOD OF DIAGNOSING APOB POLYMORPHISM CAUSING LETAL GENETIC DEFECT OF HOLSTEIN CATTLE HCD - LACK OF CHOLESTEROL

摘要

FIELD: biotechnology.;SUBSTANCE: method for determining the polymorphism of genes associated with a lethal recessive genetic defect in cattle is described. Specifically, the gene for apolipoprotein B (APOB) associated with the HCD haplotype. The polymorphism of the APOB gene associated with the HCD haplotype is determined, using a specific, single-probe polymerase chain reaction method. Herewith the amplification products of alleles A and N differ in length - a fragment of length 215 bp corresponds to allele A, a fragment of length 327 bp corresponds to allele N, and identification of animal genotypes is carried out by results of electrophoresis of PCR products in an agarose gel.;EFFECT: method can be used in farm animals genetics to detect polymorphism in the cattle APOB gene, associated with HCD fertility haplotype, in order to use the results in population genetics, cattle breeding, and selection.;1 dwg, 1 ex