IN VIVO COLLECTION AND LOCALIZED QUANTIFICATION AND PROFILING OF CIRCULATING CELLS, PROTEINS AND NUCLEIC ACIDS

摘要

The present invention relates to localized multiplex detection of RNA or proteins in situ within circulating cells that are captured in vivo by a collection device, or detection of free circulating nucleic acids captured by the same device. The device comprises a functional, three-dimensional capture surface equipped with detection receptors and the molecular assays are based on highly specific padlock probe ligation or proximity ligation assay (PLA) followed by rolling circle amplification (RCA).