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HIGHLY SPECIFIC AND SENSITIVE METHODS FOR DETECTING ENTEROHEMORRHAGIC ESCHERICHIA COLI SEROTYPES O157:H7 AND/OR O145:H28

机译：检测肠出血性大肠杆菌O157：H7和/或O145：H28血清型的高特异性和灵敏方法

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摘要

The present invention relates to methods and kits for the specific detection of Escherichia coli (E.coli) serotypes O157:H7 and/or O145:H28. The methods and kits are based on the values for the E. coli O157:H7 assay on E. coli O157:117 detection of newly identified sequence regions, which have a very high sequence identity between E.coli serotypes O157:H7 and O145:H28 and which are not present in any other known E.coli serotype or bacteria. This sequence region thus allows for selective detection of E.coli O157:1-17 and/or O145:H28 from other bacteria, especially other E.coli serotypes. Furthermore the present invention shows that a 3 bp InDel sequence in O157:H7 allows for distinguishing between O157:H7 and O145:H28, which allows for selective detection of O157:H7 over O145:H28 and vice versa. Furthermore, the invention provides oligo-nucleotides useful for said detection.

机译：本发明涉及特异性检测O157：H7和/或O145：H28血清型大肠杆菌（I.coli）的方法和试剂盒。方法和试剂盒基于 E的值。 E进行大肠杆菌 O157：H7检测。大肠杆菌 O157：117检测新鉴定的序列区域，这些序列区域在大肠杆菌血清型O157：H7和O145：H28之间具有非常高的序列同一性，并且在其他任何已知的序列区域中都不存在大肠杆菌血清型或细菌。因此，该序列区域允许选择性检测来自其他细菌（尤其是其他大肠杆菌血清型）的大肠杆菌 O157：1-17和/或O145：H28。此外，本发明显示O157：H7中的3bp InDel序列允许区分O157：H7和O145：H28，这允许相对于O145：H28选择性检测O157：H7，反之亦然。此外，本发明提供了可用于所述检测的寡核苷酸。 展开▼

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公开/公告号US2017362637A1

专利类型

公开/公告日2017-12-21

原文格式PDF

申请/专利权人 QIAGEN GMBH;
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申请/专利号US201515532558

发明设计人 SASCHA STRAUSS;KATHRIN WOLF;
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申请日2015-12-03

分类号C12Q1/68;

国家 US

入库时间 2022-08-21 13:03:22

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