首页> 外国专利> PROBES FOR IDENTIFYING GEOGRAPHICAL DISTRIBUTION AND MOLECULAR EPIDEMIOLOGY OF VIRAL HEMORRHAGIC SEPTICEMIA VIRUS (VHSV) AND USES THEREOF

PROBES FOR IDENTIFYING GEOGRAPHICAL DISTRIBUTION AND MOLECULAR EPIDEMIOLOGY OF VIRAL HEMORRHAGIC SEPTICEMIA VIRUS (VHSV) AND USES THEREOF

机译:鉴定病毒性出血性癫痫病毒(VHSV)的地理分布和分子流行病学的问题及其用途

摘要

The present invention relates to a single-nucleotide polymorphism (SNP) for determining genotypes specific to the regions from which VHSV has been isolated, to PNA for identifying same and a method for identifying, by using same, the single-nucleotide polymorphism (SNP) for determining genotypes specific to the regions from which VHSV has been isolated and, more specifically, to PNA and a kit capable of detecting the single-nucleotide polymorphism mutations of C755A and A756G of the VHSV G-protein by using PNA comprising a sequence of SEQ ID NO: 1. The present invention enables an easy, rapid and accurate identification of genotypes specific to the regions from which VHSV has been isolated, by using PNA having an excellent binding affinity to DNA so as to make each genotype exhibit a different melting temperature.
机译:本发明涉及一种用于确定特定于已分离出VHSV的区域的基因型的单核苷酸多态性(SNP),用于鉴定其的PNA,以及使用其鉴定单核苷酸多态性(SNP)的方法。用于确定特异于已分离出VHSV的区域的基因型,更具体而言,用于确定PNA的基因型,以及通过使用包含SEQ序列的PNA能够检测VHSV G蛋白的C755A和A756G的单核苷酸多态性突变的试剂盒ID NO:1。本发明通过使用对DNA具有优异的结合亲和力的PNA,使得每种基因型表现出不同的解链温度,从而能够容易,快速,准确地鉴定出从中分离出VHSV的区域特异的基因型。 。

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