Cell Separation based on Particle paramagnu00c9ticas by Continuous Flow and Particle paramagnu00c9ticas Elimination

摘要

Systems and methods are disclosed for the continuous Flow separation of cells connected to paramagnetic particles in a cell suspension containing both bound and Unbound cells.In addition, Systems, and methods to eliminate the paramagnetic particles of paramagnetic particles or cells connected to a suspension cell not linked.In addition,Disclosed is a Magnetic Separation module for continuous Flow extraction / Separation Magnetic Separation by extraction Module and a Magnetic rotating Membrane by Continuous Flow.The Cellular Processing System comprises: at least one Module of suspension CellAt least one Module of buffer; at least one module by Magnetic Separation Magnetic Separation by Continuous Flow extraction / Production Module; at least not Magnetic; and at least one ModuleProduction of Magnetic.The module of Magnetic Separation by Continuous Flow Separation by extraction / magnetic comprises: a Chamber defined by Walls and has an x direction,Direction and Z direction; an Inlet and an outlet disposed at opposite ends of the camera in the direction; and at least two adjacent Magnets or next to a Wall of the Chamber and dispueSteps to establish a line of zero gradient within the Chamber between the inlet and Outlet.The separation by extraction Module includes: a rotating Magnetic Membrane Separation Chamber by Magnetic extraction partially defined by a Cylindrical side wall; a MembranTo have a rotating Porous interior and is oriented in a coaxial with the Cylindrical side wall; a sample Inlet; a Waste Production Module connected with the Interior of theRotary Membrane Module; a Production Cell connected with the separation Chamber by Magnetic extraction; and at least one Magnet adjacent to or near the Cylindrical side wall.