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Methods and ingredients for administration and / or enhancement of herbicide tolerance, using proton oxidase or variants thereof

机译:使用质子氧化酶或其变体来施用和/或增强除草剂耐受性的方法和成分

摘要

1. Characteristic 1: polypeptide to be selected from the following: a polypeptide composed of a series of amino acids, at least one of which is to be selected from the amino acid sequence No. 2 of the stock exchange of n59, S60, R89, F161, V165, A167, q184, p303, V305, f324, l327, i340, F360 and i408, When considered separately, it is either phased out or replaced by an amino acid selected from the M (methyl) group.(in USD)I (Ile)T (Thr)L (Leu)C (Cys)A (Ala)Yes, it is.F (PE),P (Pro)W (Trp)N (Asp)Q (Gln)G (Gly)(Thai)D (Asp)E (Glu)R (Arg)H (His)y K (Lys)It is different from the original amino acids in wild animals; it is a polypeptide, including a series of amino acids, at least one of which can be selected from monomer amino acids containing R101, f171, v175, a77, g194, p316, v318, f337, l340, i353 and f373. When considered separately, it is either phased out or replaced by an amino acid selected from the M (methyl) group.(in USD)I (Ile)T (Thr)L (Leu)C (Cys)A (Ala)Yes, it is.F (PE),P (Pro)W (Trp)N (Asp)Q (Gln)G (Gly)(Thai)D (Asp)E (Glu)R (Arg)H (His)y K (Lys)In the corresponding position of wild animals, it is different from amino acids; it is a polypeptide, which is composed of a series of amino acids, with 95% or more of the amino acid sequence of these polypeptides. Claim 5: a polynucleotide that encodes polypeptides according to claims 1 to 4 of any one. Claim 8: ingredients that give or enhance the tolerance of plant or algal herbicides, including at least one ingredient selected from the polymer peptide specified by us No. 2 and the polymer specified by us No. 4;1. Polypeptides according to any one of requirements 1 to 4; polynucleotide encoded for polypeptides; recombinant vectors including polynucleotide; and recombinant cells including recombinant vectors. Requirement 16: Herbicide resistant, clonal or offspring resistant plant or algal alternators, including peptides classified according to ID 2 and peptides classified according to ID 4,Polypeptide or coding polynucleotide contained in any one of 1.1 to 4 requirements. Claim 19: a method of giving or enhancing tolerance to a plant or algal herbicide, provided that such a method includes altering the algae or plant cells, protozoa, cocoon, puffer fish, seeds, cannula, vatargo or all polypeptide plants described in Section 2, polypeptide plants described in Section 4, Polypeptide or coding polynucleotide contained in any one of 1.1 to 4 requirements. Claim 23: a method for removing unwanted aquatic organisms from a culture medium,Considering that the method includes: providing polypeptides described in part II, polypeptides described in part IV, polypeptides described in parts 1 to 4 or coding polynucleotide to an algal growth environment, In addition, the herbicide was used in the culture medium.
机译:1.特征1:选自以下的多肽:由一系列氨基酸组成的多肽,其中至少一个选自n59,S60,R89的证券交易所的2号氨基酸序列,F161,V165,A167,q184,p303,V305,f324,l327,i340,F360和i408,单独考虑时,要么被淘汰,要么被选自M(甲基)基团的氨基酸取代。 I(Ile)T(Thr)L(Leu)C(Cys)A(Ala)是,是F(PE),P(Pro)W(Trp)N(Asp)Q(Gln)G(Gly) (泰国)D(Asp)E(Glu)R(Arg)H(His)y K(Lys)它不同于野生动物中的原始氨基酸;它是包含一系列氨基酸的多肽,其中至少一个可以选自包含R101,f171,v175,a77,g194,p316,v318,f337,l340,i353和f373的单体氨基酸。当单独考虑时,它要么被淘汰,要么被选自M(甲基)基团的氨基酸取代。(美元)I(Ile)T(Thr)L(Leu)C(Cys)A(Ala) F(PE),P(Pro)W(Trp)N(Asp)Q(Gln)G(Gly)(泰)D(Asp)E(Glu)R(Arg)H(His)y K( Lys)在野生动物的相应位置,与氨基酸不同;它是一种多肽,由一系列氨基酸组成,具有这些多肽的95%或更多的氨基酸序列。权利要求5:编码根据任何一项的权利要求1-4的多肽的多核苷酸。权利要求8:赋予或增强植物或藻类除草剂耐受性的成分,包括至少一种选自美国专利2指定的聚合物肽和美国专利4; 1指定的聚合物的成分。 5.根据要求1至4中任一项的多肽;为多肽编码的多核苷酸;重组载体,包括多核苷酸;重组细胞,包括重组载体。要求16:抗除草剂,抗克隆或后代的植物或藻类交流发电机,包括按ID 2分类的肽和按ID 4分类的肽,多肽或编码1.1至4中任何一项所要求的多核苷酸。 19.提供或增强对植物或藻类除草剂的耐受性的方法,条件是该方法包括改变藻类或植物细胞,原生动物,茧,河豚,种子,套管,瓦塔戈或第2节中描述的所有多肽植物,第4节中所述的多肽植物,多肽或1.1至4要求中任何一项所要求的编码多核苷酸。 23.一种从培养基中去除不需要的水生生物的方法,考虑到该方法包括:向藻类生长环境提供第二部分所述的多肽,第四部分所述的多肽,第一部分至第四部分所述的多肽或编码多核苷酸,另外,在培养基中使用了除草剂。

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