Development of indirect imunoenzimática technique for the diagnosis of toxoplasmosis goats by detecting specific immunoglobulin G anti glycoprotein anchored to glicofosfatidilinositoL of the membrane of the protozoan parasite Toxoplasma gondii taquizoíto

摘要

The present invention patent (IP) refers to a process for the development of technical and / or process and / or the reaction of indirect immunoenzymatic test (elisai) using preferentially glicopRoteínas purified from membrane surface as taquizoíta of protozoan Toxoplasma gondii as an antigen specific and effective for the diagnosis of toxoplasmosis goats.As a distinguishing characteristic of process / technique proposed stands out the important effect to diagnose goats infected with Toxoplasma gondii, more specifically the speed.The lower cost, the accuracy and the ability to diagnose various animals at the same time when compared to other techniques available to the state of the art.It is still as important differential use of purified glycoproteins of membrane of the parasite.The development of the said kit and / or technical and / or process diagnosis may involve 7 steps.Namely: (1) the adsorption of glyc; (2) block; (3) the placing of sera; (4) placing the substrate; (5) the placing of the conjugate; (6) blocking; (7) reading.The process is specifically in the field of veterinary medicine and may contribute in a direct way for control and diagnosis of toxoplasmosis goats individually or in aFlocks.