GENE EXPRESSION MODULATION SYSTEM, EXPRESSION VECTOR, HOST CELL AND NON-HUMAN ORGANISM, AND MODULATION METHOD OF GENE EXPRESSION IN HOST CELL

摘要

gene expression modulation system; isolated polynucleotide encoding a group h nuclear receptor ligand binding domain comprising at least one mutation; expression vector; isolated host cell; isolated polypeptide comprising a group h nuclear receptor ligand binding domain comprising at least one mutation; method for modulating expression of a gene in a host cell; isolated host cell; and non-human organism. The present invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the area of gene expression. more specifically, the present invention relates to unusual substitution mutant receptors and their use in a nuclear receptor-based inducible gene expression system and methods of modulating gene expression in a host cell for applications such as gene therapy , large scale protein and antibody production, cell-based ultra-high production screening assays, functional genomics and regulation of the traits of transgenic organisms.