Method for assessment of the total DNA contents and the diagnostic test for measurements of the total DNA contents in vegetal and animal materials by spectrophotometric method

摘要

the object of the application is the evaluation of the total dna content and a diagnostic test for measuring the total dna content in plant material and animal method falsely.this method is characterized by the fact that in the first stage of preparing samples of biological material for dyeing by placing them in the 2.5% solution glutardialdehydu in 0.in ph 7.4 phosphate buffer 01 m at room temperature, after which the material washed 3-4 times in distilled water.then the tints to biological material using ethidium bromide.and then in the 80% methanol solution carried out extraction associated with dna ethidium bromide initially at room temperature, and then in cieplarce at favourably about 80°c.then the samples are wstrząsaniu at room temperature and wirowaniu, and in the final stage, the supernatant from each of the samples is used to measure the absorbance at 517 nm maximum.the test includes a sample of biological material, buffer, fixative, ethidium bromide solution and solution of 80% methanol, spectrophotometer equipped with a beam of visible light.heater and centrifuge.